dna clean & concentrate kit Search Results


vahtstm dna clean beads  (Vazyme Biotech Co)
99
Vazyme Biotech Co vahtstm dna clean beads
Vahtstm Dna Clean Beads, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna clean concentratortm  (Zymo Research)
99
Zymo Research dna clean concentratortm
Dna Clean Concentratortm, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zr dna sequencing clean uptm kit  (Zymo Research)
97
Zymo Research zr dna sequencing clean uptm kit
Zr Dna Sequencing Clean Uptm Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna clean concentrator kit  (Zymo Research)
99
Zymo Research dna clean concentrator kit
Dna Clean Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chip dna clean concentrator kit  (Zymo Research)
99
Zymo Research chip dna clean concentrator kit
Chip Dna Clean Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chip dna clean concentrator kit - by Bioz Stars, 2026-05
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nucleospin gdna clean  (MACHEREY NAGEL)
96
MACHEREY NAGEL nucleospin gdna clean
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Nucleospin Gdna Clean, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
nucleospin gdna clean - by Bioz Stars, 2026-05
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pcr clean  (Beyotime)
99
Beyotime pcr clean
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Pcr Clean, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr clean/product/Beyotime
Average 99 stars, based on 1 article reviews
pcr clean - by Bioz Stars, 2026-05
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favorprep total dna mini kit  (Favorgen Biotech)
96
Favorgen Biotech favorprep total dna mini kit
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Favorprep Total Dna Mini Kit, supplied by Favorgen Biotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/favorprep total dna mini kit/product/Favorgen Biotech
Average 96 stars, based on 1 article reviews
favorprep total dna mini kit - by Bioz Stars, 2026-05
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dna clean concentrator  (Zymo Research)
99
Zymo Research dna clean concentrator
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Dna Clean Concentrator, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna clean concentrator/product/Zymo Research
Average 99 stars, based on 1 article reviews
dna clean concentrator - by Bioz Stars, 2026-05
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dna clean and concentrator kit  (Zymo Research)
96
Zymo Research dna clean and concentrator kit
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Dna Clean And Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna clean and concentrator kit/product/Zymo Research
Average 96 stars, based on 1 article reviews
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dna clean concentrator magbead kit  (Zymo Research)
94
Zymo Research dna clean concentrator magbead kit
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
Dna Clean Concentrator Magbead Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d5207 critical  (Zymo Research)
93
Zymo Research d5207 critical
a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The <t>genomic</t> <t>DNA</t> used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .
D5207 Critical, supplied by Zymo Research, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The genomic DNA used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .

Journal: Communications Biology

Article Title: Improvement of CRISPR/Cas9 system by transfecting Cas9-expressing Plasmodium berghei with linear donor template

doi: 10.1038/s42003-020-01138-2

Figure Lengend Snippet: a The pbcas9 parasites were transfected with a linear donor template (green box) and a plasmid containing the sgRNA, resulting in a mutation of the imc1i gene. Top: HDR possibly occurred between the linear donor template with the mutation (asterisk) and the cleaved imc1i locus during the first round of the cell cycle after transfection. In addition, there were no unexpected single crossover recombinations in the resultant imc_mut_L. Middle: when the double-strand break at the target genomic locus was not repaired by HDR during the first round of the cell cycle after transfection, the parasite died due to the instability of chromosome. Bottom: when the target genomic locus was not cleaved during the first round of the cell cycle after transfection, the linear donor template was lost from the parasite and the daughter parasites would have thus only the plasmid containing sgRNA. These parasites eventually died during further cell cycles. b The genotyping PCR of imc_mut_L was performed using the sets of primers indicated at the bottom. c Southern hybridization analysis of imc_mut_L was carried out using the donor template as the DNA probe. The genomic DNA used for this analysis was purified from the transfected parasite population before cloning by limiting dilution. d A 41-bp sequence of the imc1i gene was deleted by transfecting pbcas9 with a linear donor template (green). e The imc_Δ41 parasites formed abnormal ookinetes. f The mNG gene was incorporated at the C-terminus of MTIP. g The mNG signal was detected in the pellicule of mtip::mNG ookinetes. h When pbcas9 were transfected with only the plasmid containing the sgRNA for MTIP, they died due to instability of the cleaved genome (black lines). In contrast, transgenic parasites were readily obtained using the linear donor template and the plasmid containing the sgRNA (blue lines). The uncropped images of gel or blot are shown in Supplementary Fig. .

Article Snippet: The obtained genomic DNA was further purified using the Nucleospin gDNA Clean-up kit (Macherey-Nagel).

Techniques: Transfection, Plasmid Preparation, Mutagenesis, Hybridization, Purification, Cloning, Sequencing, Transgenic Assay